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Whole Effluent Toxicity Testing

For the past 32 years, EA scientists have been involved in the design, refinement, and validation of the promulgated U.S. Environmental Protection Agency (EPA) methodologies. Our WET testing services include the following:

  • Acute toxicity tests (static, renewal, and continuous-flow) with fish, amphibians, invertebrates, and plants
  • Short-term chronic toxicity tests with fish and invertebrates
  • Full life cycle (chronic) studies with fish, DaphniaCeriodaphnia, and Americamysis species
  • Algal growth inhibition studies
  • Effluent treatability studies
  • Toxicity assessment of stormwater discharges.

Principal Whole Effluent Toxicity Testing Methods

Acute Toxicity

EPA-821-R-02-012 Methods for Measuring Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms (5th Edition); October 2002

  • Ceriodaphnia dubia – Method No. 2002.0
  • Daphnia magna and Daphnia pulex – Method No. 2021.0
  • Pimephales promelas (Fathead minnow) – Method No. 2000.0
  • Americamysis bahia – Method No. 2007.0
  • Menidia beryllina – Method No. 2006.0
  • Cyprinodon variegatus – Method No. 2004.0
  • Oncorhynchus mykiss – Method No. 2019.0.

Chronic Toxicity

EPA-821-R-02-013 Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Water to Freshwater Organisms (4th Edition); October 2002

  • Fathead minnow, Pimephales promelas, Larval Survival and Growth Test, Method No. 1000.0
  • Daphnid, Ceriodaphnia dubia, Survival and Reproduction Test, Method No. 1002.0
  • Green alga, Selenastrum capricornutum, growth, Method No. 1003.0.

EPA-821-R-02-014 Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Water to Marine and Estuarine Organisms (3rd Edition); October 2002

  • Inland silverside, Menidia beryllina (Larval Survival and Growth Test) – Method No. 1006.0
  • Mysid, Americamysis bahia (Survival, Growth, and Fecundity Test) – Method No. 1007.0
  • Sheepshead minnow, Cyprinodon variegates (Larval Survival and Growth Test) – Method No. 1004.0
  • Sea urchin, Arbacia punctulata (Fertilization) – Method No. 1008.0.

EPA-600-R-95-136 Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to West Coast Marine and Estuarine Organisms; August 1995

  • Atherinops affinis (topsmelt) (Survival and Growth) – Method No. 1006.0
  • Holmesimysis costata (Survival and Growth) – Method No. 1007.0
  • Mytilus sp. (mussel) (Shell Development) – Method No. 1008.0
  • Strongylocentrotus sp. (Urchin) (Fertilization) – Method No. 1008.0.

Contact Us

EA Engineering, Science, and Technology, Inc., PBC headquarters


Office Locations

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745 W 4th Avenue
Suite 425
Anchorage, AK 99501
Mike Jones P: 907-334-3909 Email Mike


3544 International Way
Fairbanks, AK 99701
Travis Hines P: 907-646-0210 Email Travis



700 Research Center Blvd
Suite 2221
Fayetteville, AR 72701
Matthew Frigon, PhD P: 479-575-2136 Email Matthew



555 University Avenue
Suite 182
Sacramento, CA 95825
Sheena Styger P: 916-604-4365 Email Sheena



7995 E Prentice Ave.
Suite 206E
Greenwood Village, CO 80111
Nathan Jones P: 303 590-9140 Email Nathan



131 Continental Drive
Suite 203
Newark, DE 19713
Kathy Fox P: 302-467-3025 Email Kathy


Warner Robins

108 Byrd Way
Suite 600
Warner Robins, GA 31088-9199
Tommy Bruhn P: 478-308-7375 Email Tommy


1001 Army Drive
Suite 103
Barrigada, GU 96913-1402
Billy Janasak P: 671-646-5231 Email Billy



615 Piikoi Street
Suite 515
Honolulu, HI 96814
Chip Brown P: 808-589-1455 Email Chip



444 Lake Cook Road
Suite 18
Deerfield, IL 60015
Joe Vondruska P: 847-945-8010 Email Joe


Corporate Headquarters

225 Schilling Circle
Suite 400
Hunt Valley, MD 21031
Ian MacFarlane P: 410-584-7000 Email Ian

Ecotoxicology & Biology Labs

231 Schilling Circle
Hunt Valley, MD 21031
Michael Chanov P: 410-584-7000 Email Michael


1311 Continental Drive
Suite K
Abingdon, MD 21009
Scott Dobson P: 410-671-6051 Email Scott

Ocean Pines

11200 Racetrack Road
Unit A101
Ocean Pines, MD 21811
Darl Kolar P: 410-641-5341 Email Darl



10321 E. Grand River Ave.,
Suite 600,
Brighton, MI 48116
Arthur Peitsch P: 810-206-3042 Email Arthur



54 North Last Chance Gulch
Suite 14
Helena, MT 59601
Catherine LeCours P: 406-936-0022 Email Catherine



221 Sun Valley Blvd
Suite D
Lincoln, NE 68528
Dale Schlautman P: 402-476-3766 Email Dale


7836 Wakeley Plaza
Omaha, NE 68114
Craig Johnson P: 402-817-7664 Email Craig

New Mexico


320 Gold Avenue, SW
Suite 1300
Albuquerque, NM 87102
Jay Snyder P: 505-224-9013 Email Jay

New York


P.O. Box 144
25 Western Avenue, 2nd Floor
Marlboro, NY 12542
Melanie Dina P: 845-565-8100 Email Melanie


82-B Creamery Road
Oswego, NY 13126
Sarah Nelson P: 315-342-1419 Email Sarah


Washington Station
333 West Washington Street, Suite 300
Syracuse, New York 13202
Bob Casey P: 315-431-4610 Email Bob

Rhode Island


301 Metro Center Blvd
Suite 102
Warwick, RI 02886-3440
Ron Mack P: 401-736-3440 Email Ron



405 State Highway 121 (Bypass)
Building C, Suite 100
Lewisville, TX 75067
Roger Place P: 972-315-3922 Email Roger


11550 Cypress N Houston Rd
Cypress, TX 77429
John Bonner P: 832-912-4038 Email John

San Antonio

13750 San Pedro Ave.
Suite B10
San Antonio, TX 78232
Brian O'Ferrall P: 210-546-2129 Email Brian


Virginia Beach

4429 Bonney Road
Suite 430
Virginia Beach, VA 23462
Chris Penny P: 757-693-6014 Email Chris



2200 6th Avenue
Suite 707
Seattle, WA 98121
Jen Martin Bouchard P: 401-287-0376 Email Jen

EA Satellite Residential locations (Contact 1-800-777-9750)

Dunedin, FL

Rick Hanoski

North Salem, IN

Matthew Bowman

Dover, NH

Amy Hunt

Spencerport, NY

Caryn Kiehl-Simpson

Lubbock, TX

Danny Leaks